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JD Gardner, KW Liechty and RD Christensen
Division of Human Development and Aging, University of Utah School of
Medicine, Salt Lake City 84132.
Effects of interleukin-6 (IL-6) on cycling status and clonogenic maturation
of human fetal (cord blood) and adult hematopoietic progenitors were
compared. Adult marrow cells were incubated for various lengths of time
with various concentrations of IL-6, in a serum- free system, after which
tritiated thymidine suicide studies were performed. After incubation of 2
to 5 x 10(6) cells/mL for 4 hours in 5.0 ng IL-6/mL, increased thymidine
suicide rates were observed for multipotent progenitors (CFU-Mix),
granulocyte-macrophage progenitors (CFU-GM), and erythroid burst-forming
units (BFU-E). Similar incubations of fetal cells in IL-6 resulted in
similar increases in tritiated thymidine suicide rates. In other studies,
IL-6 used alone did not support colony formation from adult progenitors.
However, it did support colony formation from fetal CFU-Mix (P less than
.05), CFU- GM (P less than .001), and BFU-E (P less than .05). In cultures
of adult progenitors, IL-6 acted synergistically with IL-3 to support CFU-
Mix colony formation (P less than .001), but synergistic actions on CFU- GM
and BFU-E were not seen. In contrast, IL-6 acted synergistically with IL-3
and with GM-CSF to support colony formation by fetal CFU-Mix, CFU-GM, and
BFU-E. Thus, IL-6 appears to have a wider spectrum of action on fetal
progenitors from cord blood than on adult progenitors; including not only
the induction of cycling, but also the support of clonogenic maturation of
CFU-Mix, CFU-GM, and BFU-E.
This article has been cited by other articles:
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| Copyright © 1990 by American Society of Hematology Online ISSN: 1528-0020 | |||||||||
