Expression of shared idiotypes by paraproteins from patients with multiple
myeloma and monoclonal gammopathy of undetermined significance
JR Berenson, A Lichtenstein, S Hart, D Palomares and RA Miller
Department of Medicine, Wadsworth Cancer Center, UCLA School of Medicine
90024.
Twenty-nine murine monoclonal antibodies have been produced that react with
shared idiotypes expressed by B-cell lymphomas and leukemias. We tested
this panel of antibodies for reactivity with the paraproteins from 32
patients with multiple myeloma and 10 patients with monoclonal gammopathy
of undetermined significance (MGUS). Thirteen of 42 paraproteins reacted
with at least one antibody in this panel of anti- idiotypic antibodies. Six
different anti-idiotypes demonstrated reactivity with the paraproteins. A
similar frequency of reactivity was found for both myeloma and MGUS
proteins. One antibody, S30-47, reacted with 6 of 32 (19%) of the
paraproteins from patients with multiple myeloma, whereas this
anti-idiotype only bound to 3% of non-Hodgkin's B- cell lymphomas and no
cases of chronic lymphocytic leukemia. This anti- idiotype reacted with
both components of a biphenotypic paraprotein (IgG kappa and IgG lambda) in
one patient. In each of nine patients tested, plasma cells isolated from
bone marrow were shown to be reactive with the same anti-idiotype we found
to react with the paraprotein. Antishared idiotype antibodies may provide
useful reagents for studies of patients with monoclonal gammopathies.
Volume 75,
Issue 11,
pp. 2107-2111,
06/01/1990
Copyright © 1990 by The American Society of Hematology
