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In situ-generated thrombin is the only enzyme that effectively activates
factor VIII and factor V in thromboplastin-activated plasma
J Pieters, T Lindhout and HC Hemker
Department of Biochemistry, University of Limburg, Maastricht, The
Netherlands.
We investigated the activation of the nonenzymatic protein cofactors factor
VIII and factor V in plasma when coagulation was initiated by
thromboplastin. With sensitive bioassays, we were able to measure
specifically the generation of activated factor VIII and activated factor V
in plasma. Our results showed that when plasma was triggered with a
relatively high concentration of thromboplastin, factor VIII and factor V
were completely activated at the clotting time of plasma. However, when the
generation of thrombin, but not that of factor Xa, was delayed by addition
of hirudin to the plasma, factor Va was generated only at the time thrombin
generation overcame the hirudin inhibition. In addition, generation of
factor VIIIa correlated with thrombin generation and not with factor Xa
generation. Furthermore, addition of large amounts of factor Xa to
hirudinized plasma did not show detectable factor VIII or factor V
activation. We concluded that in plasma activated with thromboplastin the
enzyme responsible for activation of factor V and factor VIII is thrombin,
not factor Xa.
Volume 74,
Issue 3,
pp. 1021-1024,
08/15/1989
Copyright © 1989 by The American Society of Hematology
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