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Expression of protooncogene c-myb in normal human hematopoietic cells
MB Kastan, DJ Slamon and CI Civin
Johns Hopkins Oncology Center, Baltimore, MD 21205.
Expression of the protooncogene, c-myb, in various subpopulations of normal
human hematopoietic cells was characterized. Cells expressing the immature
cell surface marker, CD34 (My10), were isolated by immune adherence with
the "panning" technique or immunomagnetic microspheres and were shown to be
strongly positive for c-myb protein expression in an immunoperoxidase
assay. The CD34+ progenitor cell population was further separated into
myeloid plus erythroid progenitors (CD34+, CD10- ) v B-lymphoid precursors
(coexpressing CD34 and CD10) by two-color FACS. Both CD34+ progenitor cell
subsets strongly expressed c-myb protein by the immunoperoxidase assay. A
flow cytometric assay was then developed which permitted simultaneous
detection of a cell-surface antigen (to characterize lineage and stage of
maturation) and the nuclear oncoprotein. This assay confirmed that CD34+
cells were strongly positive for c-myb expression and also allowed
quantitative comparisons of c-myb expression in selected populations of
other normal hematopoietic cells. Most human bone marrow cells appear to
express some level of c-myb protein, although the CD34+ progenitor cell
population expresses the highest amount.
Volume 73,
Issue 6,
pp. 1444-1451,
05/01/1989
Copyright © 1989 by The American Society of Hematology
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