|
|
 Previous Article | Table of Contents | Next Article 
Characterization of the potentiation effect of activin on human erythroid
colony formation in vitro
J Yu, L Shao, J Vaughan, W Vale and AL Yu
Department of Molecular and Experimental Medicine, Research Institute of
Scripps Clinic, La Jolla, CA 92037.
Activin, also named FSH-releasing protein, was previously shown to induce
hemoglobin accumulation in K562 cells and potentiate the proliferation and
differentiation of CFU-E in human bone marrow cultures. Present studies
indicate that the potentiation effect of activin is lineage specific. In
addition to CFU-E, activin caused an increase in the colony formation of
BFU-E from either bone marrow or peripheral blood. It had little effect on
the colony formation of CFU- GM and the mixed colonies from CFU-GEMM. In
serum-depleted culture, the effect of activin was shown to be
dose-dependent with doses effective at picomolar concentrations. The
potentiation effect of activin was exerted indirectly through mediation of
both monocytes and T lymphocytes. Activin was also found to increase
specifically the proportion of DNA-synthesizing erythroid progenitors from
both bone marrow and peripheral blood. It had little effect on DNA
synthesis in CFU-GM and in mitogen-stimulated lymphocytes. Addition of the
monocytes or T lymphocytes to their respective depleted subpopulations of
mononuclear cells reconstituted the enhancing effect of activin on the
colony formation and DNA synthesis of erythroid progenitors. These results
strongly suggest a specific role of activin in potentiating the
proliferation and differentiation of erythroid progenitors in vitro.
Volume 73,
Issue 4,
pp. 952-960,
03/01/1989
Copyright © 1989 by The American Society of Hematology
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
P. Florio, S. Perrone, S. Luisi, P. Vezzosi, M. Longini, B. Marzocchi, F. Petraglia, and G. Buonocore
Increased Plasma Concentrations of Activin A Predict Intraventricular Hemorrhage in Preterm Newborns
Clin. Chem.,
August 1, 2006;
52(8):
1516 - 1521.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Utsugisawa, J. L. Moody, M. Aspling, E. Nilsson, L. Carlsson, and S. Karlsson
A Road Map Toward Defining the Role of Smad Signaling in Hematopoietic Stem Cells
Stem Cells,
April 1, 2006;
24(4):
1128 - 1136.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Batova, H. Cottam, J. Yu, M. B. Diccianni, C. J. Carrera, and A. L. Yu
EFA (9-beta-D-erythrofuranosyladenine) is an effective salvage agent for methylthioadenosine phosphorylase-selective therapy of T-cell acute lymphoblastic leukemia with L-alanosine
Blood,
February 1, 2006;
107(3):
898 - 903.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Shav-Tal and D. Zipori
The Role of Activin A in Regulation of Hemopoiesis
Stem Cells,
November 1, 2002;
20(6):
493 - 500.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L.-e. Shao, M. B. Diccianni, T. Tanaka, R. Gribi, A. L. Yu, J. D. Pullen, B. M. Camitta, and J. Yu
Thioredoxin Expression in Primary T-Cell Acute Lymphoblastic Leukemia and Its Therapeutic Implication
Cancer Res.,
October 1, 2001;
61(19):
7333 - 7338.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. Zipori and M. Barda-Saad
Role of activin A in negative regulation of normal and tumor B lymphocytes
J. Leukoc. Biol.,
June 1, 2001;
69(6):
867 - 873.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
