Studies on the myeloid synergistic factor from 5637: comparison with
interleukin-1 alpha
IK McNiece, AB Kriegler and PJ Quesenberry
Department of Internal Medicine, University of Virginia, Charlottesville.
A synergistic factor that is produced by the human bladder carcinoma cell
line 5637 (SF-1) stimulates primitive bone marrow progenitor cells, termed
high proliferative-potential colony-forming cells (HPP- CFC), in the
presence of an optimal dose of macrophage colony stimulating factor
(CSF-1). Recent reports have demonstrated that interleukin-1 alpha (IL-1)
is identical to hemopoietin 1 and have suggested that IL-1 is the
synergistic factor present in 5637 conditioned medium (cm). We have
compared the ability of recombinant human IL-1 alpha and partially purified
preparations of SF-1 to synergize with optimal doses of CSF-1 to stimulate
HPP-CFC. In all experiments performed the numbers of HPP-CFC colonies
formed with IL-1 were significantly less than with SF-1. Replating
experiments demonstrated that SF-1 plus CSF-1 generated HPP-CFC (responsive
to IL-3 plus CSF-1); however, IL-1 plus CSF-1 resulted in no generation of
HPP- CFC. Multiple factor combinations of IL-1 and SF-1 with G-CSF, GM-CSF,
and CSF-1 also resulted in less HPP-CFC colony formation in cultures
containing IL-1 compared with SF-1. Incubation of SF-1 with an antibody to
IL-6 had no effect on HPP-CFC colony formation and IL-6 did not synergize
with IL-1 plus CSF-1 or SF-1 plus CSF-1. These data suggest the presence of
a factor in 5637 cm, which is distinct from G-CSF, GM- CSF, and IL-6, which
synergizes with IL-1 to produce the SF-1 effect.
Volume 73,
Issue 4,
pp. 919-923,
03/01/1989
Copyright © 1989 by The American Society of Hematology
