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Nonhematopoietic tumor cells express functional GM-CSF receptors
GC Baldwin, JC Gasson, SE Kaufman, SG Quan, RE Williams, BR Avalos, AF Gazdar, DW Golde and JF DiPersio
Division of Hematology-Oncology, UCLA School of Medicine.
Human granulocyte-macrophage colony-stimulating factor (GM-CSF) stimulates
the colony growth of myeloid progenitors in semisolid media, and enhances
the function of mature effector cells, including neutrophils, monocytes,
and eosinophils. Small cell carcinoma lines (SCCL) have properties of amine
precursor uptake and decarboxylation (APUD) cells and express high levels
of the enzyme, L-aromatic amino acid decarboxylase. We looked for possible
expression of GM-CSF receptors on nonhematopoietic cells and found specific
high-affinity binding of human GM-CSF to SCCL and to the SV40-transformed
African green monkey kidney cell line, COS. The small cell carcinoma lines
responded to GM-CSF with enhanced proliferation, and both small cells and
COS cells were found to express authentic 84,000 dalton GM-CSF receptor
protein. These findings indicate that nonhematopoietic cells can bind and
respond to GM-CSF, suggesting additional biological activities as well as
the possibility of tumor responses when GM-CSF is used therapeutically in
humans. Since preliminary clinical trials using CSFs as adjunctive
treatment in patients with solid tumors are underway, it will be important
to consider the possible responsiveness of nonhematopoietic tumor cells to
CSFs.
Volume 73,
Issue 4,
pp. 1033-1037,
03/01/1989
Copyright © 1989 by The American Society of Hematology
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