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Rare occurrence of N-ras point mutations in Philadelphia chromosome
positive chronic myeloid leukemia
SJ Collins, M Howard, DF Andrews, E Agura and J Radich
Molecular Medicine Program, Fred Hutchinson Cancer Research Center, Seattle
98104.
Point mutations of the N-ras oncogene are relatively common in acute
myelogenous leukemia (AML) cells, occurring in some 25% to 50% of patient
samples. We used a technique involving the direct nucleotide sequencing of
in vitro amplified N-ras genomic fragments to determine the frequency of
N-ras point mutations in chronic myeloid leukemia (CML) cells at various
stages of the disease. This approach will detect N-ras point mutations in a
mixed population of cells if the mutation is present in 25% or more of the
cells. We could not demonstrate any point mutation at N-ras codons 12,13 or
59-63 in any of the 44 CML cases analyzed, which included 21 blast crisis
samples. In contrast with AML N-ras point mutations are exceedingly rare in
CML.
Volume 73,
Issue 4,
pp. 1028-1032,
03/01/1989
Copyright © 1989 by The American Society of Hematology
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