A PvuII polymorphism of the bcr region in patients with hematopoietic
disorders and their families
B Opalka, U Wandl, O Kloke, C Oberle, J Koppe, N Niederle and CG Schmidt
Department of Internal Medicine, University Essen, FRG.
The BCR gene on chromosome 22 has received increasing attention because of
its involvement in the Philadelphia (Ph') translocation. For most
restriction enzymes, this locus has been found to be nonpolymorphic. Two
alleles have only been found when Taql-digested DNA is hybridized to a 5'
bcr-specific probe. We describe another two-allele polymorphism detected by
the same probe in PvuII-digested DNA. The polymorphism is characterized by
an additional PvuII site in the bcr region: this causes the appearance of
an additional band of about 2.3 kb or 2.5 kb besides a 4.8-kb fragment in
hybridizations with the 5' bcr or a 3' bcr probe. The incidence of the
second allele is very low. It has only been found in some patients with
hematopoietic malignancies and in a group of volunteers having a leukemia
patient in their families.
Volume 73,
Issue 3,
pp. 814-817,
02/15/1989
Copyright © 1989 by The American Society of Hematology
