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R Amakawa, S Fukuhara, H Ohno, S Doi, S Oguma, S Tanabe, H Yamabe, S Edamura, N Tomono and K Nasu
First Division of Internal Medicine, Faculty of Medicine, Kyoto University,
Japan.
A t(14;18) (q32;q21) chromosome translocation is closely associated with
the follicular lymphoma, which is prevalent in the United States, and the
t(14;18) causes the juxtaposition of a bcl-2 gene on chromosome 18 with an
immunoglobulin heavy-chain gene locus on chromosome 14. Genomic DNAs from
30 Japanese patients with follicular lymphoma were examined for the
molecular features by Southern blot hybridization. Using probe b for the
major breakpoint cluster region of a bcl-2 gene, the rearrangements were
detected in eight patients. Six of the eight patients had breakpoints
located within the major breakpoint region, while two had breakpoints
outside this cluster region but within the region of the 7.5-kb SstI
fragment containing the probe b sequence. In two patients, pFL-2 probe
detected the bcl-2 gene rearrangements that occurred near or within the
minor breakpoint cluster region. These ten patients had a rearranged
JH-containing fragment that migrated with the rearranged bcl-2 fragment. In
the other 20 patients, these two chromosome 18-specific DNA probes did not
detect the bcl-2 rearrangements. Compared with studies performed in the
United States, the statistical analysis indicates a significant difference
in frequency of the bcl-2 gene rearrangements near or within the major
breakpoint cluster region (P = 0.0027) and the minor breakpoint cluster
region (P = 0.029). However, the distribution difference of these events
was not significant.
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| Copyright © 1989 by American Society of Hematology Online ISSN: 1528-0020 | |||||||||
