c-myb but not c-myc suppresses the hemin-induced nonterminal expression of
hemoglobin by murine erythroleukemia cells
EV Prochownik
University of Michigan, Ann Arbor 48109.
Clonal lines of Friend murine erythroleukemia (F-MEL) cells have been
generated following transfection with c-myc or c-myb expression plasmids.
These clones produce high levels of abnormally regulated proto-oncogene
transcripts and fail to terminally differentiate in the presence of
dimethyl sulfoxide. To determine the relative levels at which the two
proto-oncogenes might exert their inhibitory effects, we asked whether
these clones could express differentiated functions in the absence of
terminal differentiation. It was found that exposure of c-myc-transfected
cells to hemin allows for the induction of hemoglobin, whereas
c-myb-transfected cells were refractory to hemin induction. It thus appears
that c-myb exerts a more globally suppressive effect on
F-MEL-differentiated functions than does c-myc and may prevent the
expression of those events that can otherwise be dissociated from the
terminally differentiated state.
Volume 73,
Issue 3,
pp. 782-786,
02/15/1989
Copyright © 1989 by The American Society of Hematology
