Identification of a leukocyte alloantigen with a high-frequency expression
in leukemia patients
R O'Connor, JG Bradley, A O'Meara and TG Cotter
Department of Biology, St. Patrick's College, Maynooth, Ireland.
In this report we describe the production and characterization of a
monoclonal antibody to the human promyelocytic leukemia cell line HL- 60.
The antibody, NC-2, is of the IgG1 subclass and precipitates a 50- Kd
protein from 125I-labeled HL-60 cells. The antigen is insensitive to
treatment with trypsin, papain, or neuraminidase. NC-2 did not react with a
number of established human cell lines, including Daudi, Molt-4, K562,
U937, KG-1, CEM, Raji, and Gash-P. Neutrophils and monocytelike cells
derived from HL-60 cells that were induced to differentiate continued to
express the antigen. NC-2 reacted with all peripheral- blood cells except
erythrocytes from eight (5%) of 150 normal individuals tested. Bone marrow
samples from patients with myelogenous leukemias were more frequently
reactive with NC-2 than were those from normal individuals (12/33 v 1/10).
Family studies indicated that the antigen was inherited in an
autosomal-dominant manner. These findings suggest that the expression of
the above alloantigen is associated with an increased incidence of
leukemia.
Volume 73,
Issue 2,
pp. 553-558,
02/01/1989
Copyright © 1989 by The American Society of Hematology
