Identification of a cellular polypeptide that distinguishes between acute
lymphoblastic leukemia in infants and in older children
SM Hanash, R Kuick, J Strahler, B Richardson, G Reaman, L Stoolman, C Hanson, D Nichols and HJ Tueche
Department of Pediatrics, University of Michigan Medical School, Ann Arbor
48109-0510.
We analyzed the polypeptide pattern of leukemic cells of infants and older
children with acute lymphoblastic leukemia (ALL), using two- dimensional
polyacrylamide gel electrophoresis (PAGE). Patterns were analyzed for the
occurrence of a previously detected cytosolic polypeptide, designated L3.
Quantitative analysis of L3 in 12 infants and 91 older children with non-T
ALL indicated lack of expression of polypeptide L3 in leukemic cells of
infants which, in most cases, expressed HLA-DR and CD19 and lacked CD10.
Quantitative analysis of L3 in relation to cell surface marker expression
revealed that L3 was limited in its occurrence to non-T ALL and was not
coordinately expressed with any of the surface markers included in the
study. Among patients in the HLA-DR-positive, CD19-positive, and
CD10-negative group, different levels of polypeptide L3 were observed
between infants and older children. These results indicate differences in
leukemic cell constituents between infants and older children with ALL and
an otherwise similar cell surface marker phenotype.
Volume 73,
Issue 2,
pp. 527-532,
02/01/1989
Copyright © 1989 by The American Society of Hematology
