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Interleukin-6 synergizes with M-CSF in the formation of macrophage colonies
from purified human marrow progenitor cells
FJ Bot, L van Eijk, L Broeders, LA Aarden and B Lowenberg
Dr Daniel den Hoed Cancer Center, Rotterdam, The Netherlands.
We examined the in vitro stimulative effects of recombinant human
interleukin-6 (IL-6, or interferon-beta 2) on purified human bone marrow
progenitor cells. IL-6 alone or in combination with erythropoietin (Epo),
IL-3, GM-CSF, or G-CSF did not induce colony formation. However, IL-6
strongly synergized with M-CSF in stimulating macrophage colony formation
(colony numbers and size). The magnitude of IL-6 synergism with M-CSF was
dose dependent; maximal potentiation of M- colony formation was evident at
approximately 100 to 1,000 U/mL IL-6. When the addition of IL-6 to
M-CSF-supplemented cultures was delayed for more than one day after the
beginning of culture, enhancement of macrophage colony formation was lost.
IL-6 stimulation of M-CSF- responsive colony formation was not apparent
when nonpurified marrow cells were plated, most likely due to endogenous
IL-6 release. These observations suggest that IL-6, in addition to playing
a role in B- lymphocyte proliferation can potentiate the human immune
defence mechanism by stimulating monocyte-macrophage development as well.
Volume 73,
Issue 2,
pp. 435-437,
02/01/1989
Copyright © 1989 by The American Society of Hematology
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