Effect of 5-fluorouracil on "primitive" hematopoietic stem cells that
reconstitute whole erythropoiesis of genetically anemic W/Wv mice
T Nakano, N Waki, H Asai and Y Kitamura
Division of Cancer Pathology, Osaka University Medical School, Japan.
The potential to reconstitute the whole erythropoiesis of a genetically
anemic (WB X C57BL/6)F1-W/Wv (WBB6F1-W/Wv) mouse for at least 8 weeks was
compared between 5-fluorouracil (5FU)-treated and nontreated bone marrow
cells. C57BL/6-Pgk-1b/Pgk-1a female mice, in which each stem cell had
either A-type or B-type phosphoglycerate kinase (PGK) owing to the random
inactivation of one of two X chromosomes, were used as donors. As a marker
of the reconstitution, electrophoretic pattern of hemoglobin was used. The
concentration of the stem cells that reconstitute the whole erythropoiesis
of WBB6F1-W/Wv mouse was higher in the marrow of donors that had received
an injection of 5FU two days previously (two-day 5FU-treated) than in the
marrow of nontreated donors. In the marrow of four-day 5FU-treated mice,
however, the concentration was comparable to that of nontreated mice. The
PGK electrophoretic pattern of WBB6F1-W/Wv mice reconstituted by nontreated
marrow cells was comparable to the PGK pattern of WBB6F1-W/Wv mice
reconstituted by four-day 5FU-treated marrow cells. Thus, a single stem
cell with extensive proliferative potential rather than multiple spleen
colony-forming units appeared to be responsible for the erythropoietic
reconstitution in the transplantation of nontreated healthy marrow cells as
well as 5FU-treated marrow cells.
Volume 73,
Issue 2,
pp. 425-430,
02/01/1989
Copyright © 1989 by The American Society of Hematology
