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Ultraviolet B light inactivates bone marrow T lymphocytes but spares
hematopoietic precursor cells
HJ Deeg, L Bazar, M Sigaroudinia and M Cottler-Fox
Bone Marrow Transplantation Program, Georgetown University, Washington, DC.
Bone marrow cells from ten normal donors were exposed to ultraviolet (UV)C
or UVB light for total exposures of 0.1 to 100 mJ/cm2, and assayed for
granulocyte-macrophage colony-forming units (CFU-GM), erythroid
burst-forming units (BFU-E), and phytohemagglutinin (PHA)- stimulated
proliferative responses. After exposure to UVC CFU-GM, BFU-E and PHA
responses showed a UV dose-dependent sharp decrease to levels less than 1%
of controls with 0.5, 2.0, and 10 mJ/cm2, respectively. With UVB, PHA
responses were most sensitive, declining to less than 1% at 5 mJ/cm2. BFU-E
decreased to less than 1% of control with 15 mJ/cm2 UVB. CFU-GM, at UVB
doses of 0.1 to 2.0 mJ/cm2, increased to 125% to 130% of control and
decreased to less than 1% only at exposures greater than 20 mJ/cm2. Thus,
these studies show that UVB, but not UVC light, can be used to inactivate
bone marrow T lymphocytes selectively while sparing hematopoietic precursor
cells. The data suggest that UVB irradiation can be used for T-lymphocyte
purging for allogeneic marrow transplantation.
Volume 73,
Issue 2,
pp. 369-371,
02/01/1989
Copyright © 1989 by The American Society of Hematology
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