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Proliferation and maturation of human erythroid progenitors in liquid
culture
E Fibach, D Manor, A Oppenheim and EA Rachmilewitz
Department of Hematology, Hadassah University Hospital, Jerusalem, Israel.
Hemopoiesis is studied in vitro mainly in semisolid culture, where
hemopoietic progenitors develop into discrete colonies. We describe a
liquid culture system that supports the proliferation and maturation of
human erythroid progenitors. We seeded mononuclear cells from the
peripheral blood (PB) of patients with beta-thalassemia in liquid medium in
the presence of conditioned medium from human bladder carcinoma cells.
Seven days later, RBCs, normoblasts, granulocytes, and monocytes
disappeared, and the number of lymphocytes dropped considerably. In
contrast, erythroid colony-forming cells increased fourfold to tenfold. The
next step entailed the removal of colony- stimulating factor (CSF) and
CSF-secreting cells, the exclusion of macrophages by harvesting nonadherent
cells, and the lysis of T lymphocytes by treatment with monoclonal rat
antihuman lymphocyte antibodies (CAMPATH-1) and complement. Reculture of
the remaining cells in liquid medium supplemented with recombinant
erythropoietin (EPO) resulted in the exclusive development of erythroid
cells, with myeloid cells reduced to less than 2%. Stainable hemoglobin
(Hb) appeared on day 3, with over 85% of the population containing
hemoglobin by day 11 and the cell number increasing from 0.2 X 10(6) to 3 X
10(6) mL. By permitting the manipulation of culture conditions and
components and increasing the cell yield, the liquid system may facilitate
quantitative analysis of growth kinetics as well as biochemical and
immunologic characterization of the developing erythroid cell.
Volume 73,
Issue 1,
pp. 100-103,
01/01/1989
Copyright © 1989 by The American Society of Hematology
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|
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