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Polyclonal reconstitution of human marrow after allogeneic bone marrow
transplantation
R Nash, R Storb and P Neiman
Fred Hutchinson Cancer Research Center, Seattle, WA 98104.
Clonal dominance suggestive of reconstitution of marrow from small numbers
of pluripotent hematopoietic stem cells has been noted in different
experimental and clinical situations. Recipients of human allogeneic marrow
transplants have not been previously studied to determine if clonal
dominance occurs in this clinical setting. Clonal analysis of 20 allogeneic
marrow transplant recipients was performed on DNA from peripheral blood
neutrophils using restriction fragment length polymorphisms on the X
chromosome. Similar studies were performed on 16 of the donors. To analyze
the results further, recipients were paired with their respective donors.
There was no evidence of shifts in cell populations contributing to the X
chromosome inactivation patterns in recipient marrow grafts when compared
with their respective donors. A mathematical model based on binomial
statistics was adapted to estimate the numbers of reconstituting
pluripotent hematopoietic stem cells. There was no evidence of clonal
dominance suggestive of oligoclonal reconstitution in marrow grafts after
allogeneic marrow transplantation. This does not preclude the possibility
of oligoclonal reconstitution in other marrow transplant settings such as
autologous transplantation.
Volume 72,
Issue 6,
pp. 2031-2037,
12/01/1988
Copyright © 1988 by The American Society of Hematology
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