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Platelets secrete a coagulation inhibitor functionally and antigenically
similar to the lipoprotein associated coagulation inhibitor
WF Novotny, TJ Girard, JP Miletich and GJ Broze
Division of Hematology/Oncology, Washington University School of Medicine,
Jewish Hospital, St. Louis, MO 63110.
Stimulation with thrombin or the calcium ionophore, A23187 caused human
platelets to release a coagulation inhibitor similar to the Lipoprotein
Associated Coagulation Inhibitor (LACI). This was documented functionally,
with clotting assays measuring tissue factor inhibition and factor Xa
inhibition, as well as immunologically, in a competitive immunoassay. The
total amount of LACI released by 3 x 10(8) platelets after two hours
stimulation was 7% to 8% of the amount found in 1 mL of serum. Half of the
LACI was released by five minutes. The LACI was present in the platelet
supernatant and was not associated with the platelet membrane or shed
vesicles. The tissue factor and factor Xa inhibitory activities that were
released were neutralized by preincubating the platelet supernatants with
specific rabbit polyclonal anti-LACI IgG. On Western blot, platelet LACI
appeared to run as a doublet with a molecular weight (mol wt) 45,000 to
47,000. Blood samples obtained from the site of a wound (template bleeding
time) demonstrated a progressive increase in LACI concentration. A cDNA
probe, derived from endothelial cell LACI cDNA, hybridized selectively to
4.0 and 1.4 kb transcripts in a preparation of platelet mRNA.
Volume 72,
Issue 6,
pp. 2020-2025,
12/01/1988
Copyright © 1988 by The American Society of Hematology
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