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Characterization of a new megakaryocytic cell line: the Dami cell
SM Greenberg, DS Rosenthal, TA Greeley, R Tantravahi and RI Handin
Hematology Division, Brigham and Women's Hospital, Boston, MA 02115.
A new human megakaryocytic cell line (Dami) has been established from the
blood of a patient with megakaryoblastic leukemia. The Dami cells grow
primarily in suspension with a doubling time of 24 to 30 hours. By light
and electron microscopy, the Dami cells range in size from 12 to 120 micron
in diameter and have lobulated nuclei characteristic of megakaryocytes. At
least 89% of the cells react with monoclonal antibodies against platelet
glycoproteins (GP) Ib and IIB/IIIa, and glycophorin. The cells do not react
with antibodies against lymphoid, monocyte, granulocyte, or macrophage
antigens. Thirteen percent of the cells become polyploid, spontaneously
achieving greater than 4N DNA ploidy levels. In response to phorbol
myristate acetate (PMA), the proportion of cells with ploidy levels greater
than 4N increased threefold and could be separated into discrete ploidy
groups. PMA also increased the expression of GPIb, the GPIIb/GPIIIa
complex,l and von Willebrand factor. Cytogenetic analysis revealed a human
male hyperdiploid karyotype with a modal chromosome number of 54 to 64 and
several consistent clonal chromosomal abnormalities. These included a
partial deletion of chromosome 5 and a translocation involving chromosome
3. In contrast to other megakaryocytic cell lines in which only a small
portion of the cells express the megakaryocytic phenotype, nearly all of
the Dami cells express platelet glycoproteins. Thus, the Dami cells provide
a superior model in which to study human megakaryocyte biochemistry and
differentiation.
Volume 72,
Issue 6,
pp. 1968-1977,
12/01/1988
Copyright © 1988 by The American Society of Hematology
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