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Detection of leukemic clone maturation in vivo by premature chromosome
condensation
WN Hittelman, P Agbor, I Petkovic, B Andersson, H Kantarjian, R Walters, C Koller and M Beran
Department of Medical Oncology, University of Texas M.D. Anderson Cancer
Center, Houston 77030.
The purpose of this study was to determine the feasibility of using the
technique of premature chromosome condensation to detect the in vivo
maturation of abnormal elements in patients with chronic myelogenous
leukemia (CML), myelodysplastic syndrome, and acute leukemia. Patients were
chosen for study if there were a clinical suggestion of in vivo maturation
and a leukemic clone exhibiting a distinguishable karyotypic abnormality.
Mature peripheral blood granulocytes were enriched by two- step
Ficoll-Hypaque gradient sedimentation and fused with mitotic Chinese
hamster ovary cells to induce the formation of prematurely condensed
chromosomes (PCC). These PCC were then analyzed for chromosome number per
cell (in the case of patients with a numerical abnormality) or by G-banding
(in the case of specific translocations). Of 13 patients chosen for study,
12 showed karyotypic evidence for maturation of the abnormal elements in
vivo. Maturation was observed in a number of clinical situations including
before treatment in benign CML and myelodysplasia, after low-dose and
high-dose chemotherapy in myelodysplasia and acute myelogenous leukemia
(AML), and in remission. These results suggest that the technique of
premature chromosome condensation can be a powerful tool in better
understanding the biology of disease and mode of response to therapy in
vivo in patients with leukemia and preleukemic syndromes, especially during
treatment with agents thought to induce maturation of the leukemic
elements.
Volume 72,
Issue 6,
pp. 1950-1960,
12/01/1988
Copyright © 1988 by The American Society of Hematology
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