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This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Henni, T. Articles by Goossens, M. Search for Related Content PubMed PubMed Citation Articles by Henni, T. Articles by Goossens, M. Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  Comparison of genetic probe with immunophenotype analysis in lymphoproliferative disorders: a study of 87 cases T Henni, P Gaulard, M Divine, JP Le Couedic, D Rocha, C Haioun, Z Henni, JP Marolleau, Y Pinaudeau and M Goossens INSERM U.91, Service d'Hematologie Clinique, Creteil, France. We examined 91 specimens (from 87 patients) for the expression of B- cell- and T-cell-associated differentiation antigens and rearrangements of the Ig and beta-chain of the T-cell (beta-TCR) genes. Of these, 74 were representative of various histologic subtypes of non-Hodgkin's lymphoma and related disorders, 11 of Hodgkin's disease, and 6 of reactive lymphoid hyperplasia. An Ig gene clonal rearrangement correlated to a monotypic (kappa/lambda) phenotype in 32 of 33 histologically defined lymphoma samples. The genotypic analysis also confirmed clonality in six of seven malignant diffuse lymphomas that were nonmonotypic but expressed pan-B antigens; in four, more than one clone was detected within individual tumors. A beta-TCR clonal rearrangement was found in 19 of 19 tumor samples considered as malignant T-cell lymphoma on the basis of histopathology and of the CD3- positive phenotype of tumoral cells, and in two cases of CD3-positive lymphomatoid disorders. A loss of pan-T antigens (CD7, CD5, CD2, CD4/CD8) was observed in all but three of these CD3-positive samples. Such an incomplete T-cell phenotype always correlated to the presence of a monoclonal process as revealed by genotypic analysis. DNA analysis was the only way to demonstrate clonality in other samples with either a polymorphous (partial involvement, pseudolymphoma, angioimmunoblastic lymphodenopathy [AILD]) or an undifferentiated (large cell anaplastic) phenotype. It is concluded that although in the majority of cases immunophenotyping alone provides criteria adequate for the diagnosis of lymphoid malignancy, in some, particularly polymorphous or large cell anaplastic processes, genetic probe analysis was additionally discriminative. Volume 72, Issue 6, pp. 1937-1943, 12/01/1988 Copyright © 1988 by The American Society of Hematology CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: C. Fournel-Fleury, F. Ponce, P. Felman, A. Blavier, C. Bonnefont, L. Chabanne, T. Marchal, J. L. Cadore, I. Goy-Thollot, D. Ledieu, et al. Canine T-cell Lymphomas: A Morphological, Immunological, and Clinical Study of 46 New Cases Vet. Pathol., January 1, 2002; 39(1): 92 - 109. [Abstract] [Full Text] [PDF]

	Copyright © 1988 by American Society of Hematology         Online ISSN: 1528-0020