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Interleukin-3 and interleukin-1 alpha allow earlier bone marrow progenitors
to respond to human colony-stimulating factor 1
YQ Zhou, ER Stanley, SC Clark, JA Hatzfeld, JP Levesque, C Federici, SM Watt and A Hatzfeld
Laboratory of Cellular and Molecular Biology of Growth Factors, Hopital
Paul Brousse, Villejuif, France.
By using human bone marrow cells enriched for early progenitors by
selective immunoadsorption and plated at low cell density (10(3) to 10(4)
cells/mL/9.6 cm2) in semisolid methylcellulose culture, we have analyzed
the cooperative effects of human colony-stimulating factor 1 (CSF-1),
granulocyte-macrophage-CSF (GM-CSF), interleukin-1 alpha (IL-1 alpha), and
gibbon as well as human recombinant IL-3 on the formation of monocytic
colonies. CSF-1 alone stimulated mature monocytic colony formation by human
CFU-M. However, in the presence of IL-3 and erythropoietin, CSF-1
stimulated maximal immature monocytic colony formation at low
concentrations and inhibited the formation of granulomonocytic,
erythrocytic, and mixed colonies. Cultures with CSF-1 and IL-3 contained
more immature monocytic colonies than did cultures with CSF-1 alone. IL-1
alpha alone had little effect. However, IL-1 alpha in combination with
optimal concentrations of either CSF-1, GM- CSF, or IL-3 increased the
number of colonies containing immature or mature monocytic colonies.
Volume 72,
Issue 6,
pp. 1870-1874,
12/01/1988
Copyright © 1988 by The American Society of Hematology
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N Fortunel, P Batard, A Hatzfeld, M. Monier, B Panterne, J Lebkowski, and J Hatzfeld
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B Panterne, A Hatzfeld, P Sansilvestri, A Cardoso, M. Monier, P Batard, and J Hatzfeld
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