Antibody produced against isolated Rh(D) polypeptide reacts with other
Rh-related antigens
K Suyama and J Goldstein
Lindsley F. Kimball Research Institute, New York Blood Center, NY 10021.
Rh(D) antigen-containing polypeptide was prepared by immune precipitation
of intact cDE/cDE erythrocytes by using a high-titer preparation of
polyclonal anti-D. when isolated Rh(D) polypeptide was administered to
rabbits, antibody was produced that was unresponsive toward Rh-positive and
-negative cells but reacted strongly with the immunogen in enzyme-linked
immunosorbent assay-type immunobinding and Western blot immunostaining
assays. Rabbit antibody also immunostains isolated Rh(c) polypeptide as
well as the Rh antigen-containing components of sodium dodecyl
sulfate-polyacrylamide gel electrophoresis- separated membrane proteins
from Rh(D)-positive (cDE/cDE,CDe/CDe), Rh(D)-negative (cde/cde,Cde/Cde),
and -D-/-D- cells. It does not react with any membrane protein from Rh-null
regulator type cells, thus indicating a specificity for Rh-related
proteins. We have also been able to demonstrate that polyclonal and
monoclonal anti-D preparations that do not immunostain isolated Rh(D)
polypeptide will react with it in our immunobinding assay.
Volume 72,
Issue 5,
pp. 1622-1626,
11/01/1988
Copyright © 1988 by The American Society of Hematology
