|
|
 Previous Article | Table of Contents | Next Article 
Platelet glycoprotein IIb/IIIa complex in cultured cells. Localization in
focal adhesion sites in spreading HEL cells
J Ylanne, M Hormia, M Jarvinen, T Vartio and I Virtanen
Department of Anatomy, University of Helsinki, Finland.
A panel of mouse monoclonal antibodies (MoAbs) was raised that react with
platelet glycoproteins (GP) IIb or IIIa. On immunofluorescence, the MoAbs
reacted with 30% to 40% of the human erythroleukemia (HEL) cells. When the
HEL cells were induced to spread on fibronectin in the presence of
12-O-tetradecanoylphorbol-13-acetate (TPA), the MoAbs reacted with the
focal adhesion sites. Only some of the GPIIIa MoAbs reacted with cells
other than platelets, megakaryocytes, or HEL cells, and these showed focal
adhesion sites in cultured human endothelial cells, fibroblasts, and
epithelial cells from normal kidney tubules. They did not react, however,
with transformed fibroblasts, fibrosarcoma cells, cultured cells from
hypernephromas, or cultured human amnion epithelial cells. The results
suggest that the platelet-type GPIIb/IIIa complex is only expressed in
cells showing an ability to define megakaryoblastic differentiation.
Localization of the GPIIb/IIIa complex at the induced focal adhesion sites
in HEL cells and localization of the GPIIIa-like molecules in other cells
suggest their direct role in the adhesion process and in the actomyocin
organization of adherent cells.
Volume 72,
Issue 5,
pp. 1478-1486,
11/01/1988
Copyright © 1988 by The American Society of Hematology
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
A. Matsumoto, T. Kamata, J. Takagi, K. Iwasaki, and K. Yura
Key Interactions in Integrin Ectodomain Responsible for Global Conformational Change Detected by Elastic Network Normal-Mode Analysis
Biophys. J.,
September 15, 2008;
95(6):
2895 - 2908.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Kamata, M. Handa, Y. Sato, Y. Ikeda, and S. Aiso
Membrane-proximal {alpha}/{beta} Stalk Interactions Differentially Regulate Integrin Activation
J. Biol. Chem.,
July 1, 2005;
280(26):
24775 - 24783.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
I. Virtanen, M. Korhonen, N. Petajaniemi, T. Karhunen, L.-E. Thornell, L. M. Sorokin, and Y. T. Konttinen
Laminin Isoforms in Fetal and Adult Human Adrenal Cortex
J. Clin. Endocrinol. Metab.,
October 1, 2003;
88(10):
4960 - 4966.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Filenius, T. Tervo, and I. Virtanen
Production of Fibronectin and Tenascin Isoforms and Their Role in the Adhesion of Human Immortalized Corneal Epithelial Cells
Invest. Ophthalmol. Vis. Sci.,
August 1, 2003;
44(8):
3317 - 3325.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Kamata, K. K. Tieu, A. Irie, T. A. Springer, and Y. Takada
Amino Acid Residues in the alpha IIb Subunit That Are Critical for Ligand Binding to Integrin alpha IIbbeta 3 Are Clustered in the beta -Propeller Model
J. Biol. Chem.,
November 16, 2001;
276(47):
44275 - 44283.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. Joki-Korpela, V. Marjomäki, C. Krogerus, J. Heino, and T. Hyypiä
Entry of Human Parechovirus 1
J. Virol.,
February 15, 2001;
75(4):
1958 - 1967.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
W. Puzon-McLaughlin, T. Kamata, and Y. Takada
Multiple Discontinuous Ligand-mimetic Antibody Binding Sites Define a Ligand Binding Pocket in Integrin alpha IIbbeta 3
J. Biol. Chem.,
March 10, 2000;
275(11):
7795 - 7802.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
X.-P. Zhang, T. Kamata, K. Yokoyama, W. Puzon-McLaughlin, and Y. Takada
Specific Interaction of the Recombinant Disintegrin-like Domain of MDC-15 (Metargidin, ADAM-15) with Integrin alpha vbeta 3
J. Biol. Chem.,
March 27, 1998;
273(13):
7345 - 7350.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Kamata, A. Irie, M. Tokuhira, and Y. Takada
Critical Residues of Integrin alpha IIb Subunit for Binding of alpha IIbbeta 3 (Glycoprotein IIb-IIIa) to Fibrinogen and Ligand-mimetic Antibodies (PAC-1, OP-G2, and LJ-CP3)
J. Biol. Chem.,
August 2, 1996;
271(31):
18610 - 18615.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Ylänne, J. Huuskonen, T. E. O'Toole, M. H. Ginsberg, I. Virtanen, and C. G. Gahmberg
Mutation of the Cytoplasmic Domain of the Integrin [IMAGE][IMAGE]Subunit
J. Biol. Chem.,
April 21, 1995;
270(16):
9550 - 9557.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
