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Chemotactic activity of recombinant human granulocyte colony- stimulating
factor
JM Wang, ZG Chen, S Colella, MA Bonilla, K Welte, C Bordignon and A Mantovani
Istituto di Ricerche Farmacologiche Mario Negri, Milan, Italy.
Recombinant human granulocyte colony-stimulating factor (rhG-CSF) induced
migration across polycarbonate filters of human polymorphonuclear
leukocytes (PMN). rhG-CSF was active in inducing PMN migration at
concentrations greater than or equal to 10 to 100 U/mL (7 to 70 ng/mL).
rhG-CSF did not contain appreciable levels of endotoxin contamination as
assessed by Limulus amebocyte assay, and Polymixin B did not affect the
chemotactic activity of rhG-CSF. A monoclonal anti-G- CSF antibody blocked
the induction of migration by G-CSF, thus establishing that the cytokine
was responsible for the activity of the recombinant preparation.
Checkerboard analysis was performed by seeding different concentrations of
G-CSF above and/or below the filter and revealed that the migratory
response to this cytokine was best observed in the presence of a positive
concentration gradient between the lower and upper compartments of the
chamber, thus indicating an actual chemotactic effect. When different
migrating cells were examined, rhG- CSF was inactive on large granular
lymphocytes and endothelial cells under conditions in which appropriate
reference attractants were active. In contrast, rhG-CSF elicited a
chemotactic response in monocytes inhibited by specific antibody. Thus,
G-CSF is a chemotactic signal for phagocytes. This cytokine, when produced
at inflammatory sites, may contribute to the recruitment of phagocytes from
the blood compartment to amplify resistance against certain noxious agents.
Volume 72,
Issue 5,
pp. 1456-1460,
11/01/1988
Copyright © 1988 by The American Society of Hematology
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