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Proliferation of myeloid progenitor cells in human long-term bone marrow
cultures is stimulated by interleukin-1 beta
WE Fibbe, HM Goselink, G Van Eeden, J Van Damme, A Billiau, PJ Voogt, R Willemze and JH Falkenburg
Department of Hematology, University Medical Center, Leiden, The
Netherlands.
To study the effect of interleukin-1 (IL-1) beta on the proliferation of
hematopoietic progenitor cells (HPC) in long-term bone marrow cultures
(LTBMC), stromal cell layers were established from normal human bone
marrow. Autologous cryopreserved mononuclear phagocyte- and
T-lymphocyte-depleted bone marrow cells were reinoculated on the stromal
layers in fresh culture medium, with or without the addition of human IL-1
beta (30 U/mL). Once a week, half of the culture supernatant was replaced
with fresh culture medium with or without IL-1, and all nonadherent cells
were returned to the flasks. At weekly intervals during a period of 5
weeks, one culture was sacrificed to determine the total number of cells
and hematopoietic progenitor cells, present in the adherent and the
nonadherent cell fractions. In IL-1-stimulated cultures, the number of
cells recovered during a period of 5 weeks exceeded the number of cells in
unstimulated control cultures by 1.5 times. This difference was attributed
to a twofold increase in the number of adherent cells. The number of HPC
recovered from IL-1- stimulated cultures was not different from that
recovered from controls. The levels of colony-stimulating activity (CSA) in
supernatants from IL-1-stimulated cultures were significantly higher than
those in supernatants from control cultures. These results indicate that
IL-1 enhances the recovery of cells in LTBMC by stimulating the
proliferation of HPC with the concurrent release of CSA from stromal cells,
without diminishing the number of HPC.
Volume 72,
Issue 4,
pp. 1242-1247,
10/01/1988
Copyright © 1988 by The American Society of Hematology
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