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C Chatelain, M De Bast and M Symann
Laboratory of Oncology and Experimental Hematology, Catholic University,
Brussels, Belgium.
Murine bone marrow cells were separated on discontinuous Percoll gradients
and assayed for their ability to give rise to megakaryocyte colonies.
Ninety-one percent of the megakaryocyte progenitors (CFU-M) sedimented at
densities between 1.070 and 1.080 g/mL. Six percent of CFU-M were found at
densities between 1.060 and 1.070 g/mL, 2% between 1.050 and 1.060 g/mL,
whereas less than 1% had a density either lower than 1.050 g/mL or higher
than 1.080 g/mL. The number of doublings and endomitoses achieved by
progenitors of density classes higher than 1.050 g/mL were similar.
However, colonies derived from CFU-M of densities less than 1.050 g/mL
(LD-CFU-M) had a higher probability of polyploidization and a lower
probability of cell division in vitro. The inverse correlation found
between the number of cells per colony and their DNA content was invariate
regardless of the density class of the progenitors. The heterogeneity of
the ploidy of cells within colonies increased continuously with increasing
cell numbers per colony. The study if a short-period exposure of LD-CFU-M
to acute thrombocytopenia could modify the ploidy of their progeny, mice
were given rabbit antimouse platelet serum while control animals received
normal rabbit serum. Twenty-four hours after injection, marrow was
cultured. After a five-day culture period, no change in the number of
colonies, doublings, ploidy, and heterogeneity of ploidy were observed
between control and thrombocytopenic animals. The data suggests that
LD-CFU-M are a distinct category of CFU-M, perhaps more mature than the
common CFU-M.
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| Copyright © 1988 by American Society of Hematology Online ISSN: 1528-0020 | |||||||||
