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Enzymatic amplification of HTLV-I viral sequences from peripheral blood
mononuclear cells and infected tissues
S Kwok, G Ehrlich, B Poiesz, R Kalish and JJ Sninsky
Cetus Corporation, Department of Diagnostic Research, Emeryville, CA 94608.
Human T-cell lymphotropic virus type I (HTLV-I) and human T-cell
lymphotropic virus type II (HTLV-II) have been associated with adult T-
cell leukemia/lymphoma (ATL) and a rare T-cell variant of hairy cell
leukemia, respectively. Direct detection of viral nucleic acid in
peripheral blood lymphocytes (PBLs) and infected tissues in carrier
patients and those with chronic disease has proven refractory due to viral
transcriptional dormancy and the small number of infected cells present.
The investigators report here the successful application of the DNA
amplification procedure, termed PCR, to the detection of these human
oncoviruses. Judicious selection of specific oligonucleotides for primers
and probes provides type-specific and simultaneous detection of these two
retroviruses. The ability to amplify and detect highly conserved regions of
these medically relevant viruses may facilitate the identification of, as
yet, uncharacterized retroviruses.
Volume 72,
Issue 4,
pp. 1117-1123,
10/01/1988
Copyright © 1988 by The American Society of Hematology
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