Establishment and characterization of an amylase-producing human myeloma
cell line
H Matsuzaki, H Hata, M Takeya and K Takatsuki
Second Department of Internal Medicine, Kumamoto University, Medical
School, Japan.
Two stable lines of IgA lambda-producing plasma cells (KHM-1A and KHM- 1B)
that were free of the Epstein-Barr virus were established from a patient
with multiple myeloma complicated by hyperamylasemia. Surface marker
studies of the two cell lines showed that the cells had no surface
immunoglobulins but were positive for cytoplasmic immunoglobulins (IgA
lambda) and for HLA-DR and PCA-1. Secretion of IgA monoclonal
immunoglobulin by the two lines was detected by a plaque- forming cell
assay and by an enzyme-linked immunosorbent assay of culture media. KHM-1B
cells also secreted alpha-amylase, but no such activity was detected in the
culture-conditioned supernatant fluid of KHM-1A.
Volume 72,
Issue 3,
pp. 978-982,
09/01/1988
Copyright © 1988 by The American Society of Hematology
