|
|||||||||
|
|
|
|
|
|
|
|
|
|
|
A Varvarigou-Frima, S Mantagos and NG Beratis
Department of Pediatrics, Medical School, University of Patras, Greece.
Since immune memory in Rh(D)-negative isoimmunized subjects remains through
life, even in the absence of measurable anti-Rh(D), we investigated the
transformation of lymphocytes from such donors by Rh(D) antigen. The time
lapse from the last stimulus was up to 13 years. Mononuclear cells from
immunized women were stimulated by Rh(D)- positive erythrocyte stroma.
Maximum transformation was observed on the sixth day of culture, with a
stroma protein concentration of 8 micrograms/mL of culture medium. The
stimulation index (SI) in cells from 11 immunized women was 6.8 +/- 3.1
(mean +/- SD), with a range from 3.1 to 15.0. In five different sets of
control cultures, the SI ranged from 0.9 +/- 0.2 to 1.3 +/- 0.4. There was
no overlap between stimulated and control cultures. No anti-D could be
demonstrated in the serum of four of the 11 immunized cases studied. Also,
transformation was observed in mononuclear cells from Rh(D)-negative
immunized women with Rh(D)-positive erythrocytes. The findings demonstrate
that lymphocytes from isoimmunized Rh(D)-negative subjects maintain the
immune memory and are transformed in vitro by the Rh(D) isoantigen.
This article has been cited by other articles:
| |||||||||||
 |
 |
 |
|||||||
 |
 |
 |
 |
 |
 |
 |
 |
||
| Copyright © 1988 by American Society of Hematology Online ISSN: 1528-0020 | |||||||||
