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Multiple bidirectional alterations of phenotype and changes in
proliferative potential during the in vitro and in vivo passage of clonal
mast cell populations derived from mouse peritoneal mast cells
Y Kanakura, H Thompson, T Nakano, T Yamamura, H Asai, Y Kitamura, DD Metcalfe and SJ Galli
Division of Cancer Pathology, Osaka University Medical School, Japan.
Mouse peritoneal mast cells (PMC) express a connective tissue-type mast
cell (CTMC) phenotype, including reactivity with the heparin-binding
fluorescent dye berberine sulfate and incorporation of [35S] sulfate
predominantly into heparin proteoglycans. When PMC purified to greater than
99% purity were cultured in methylcellulose with IL-3 and IL-4,
approximately 25% of the PMC formed colonies, all of which contained both
berberine sulfate-positive and berberine sulfate-negative mast cells. When
these mast cells were transferred to suspension culture, they generated
populations that were 100% berberine sulfate-negative, a characteristic
similar to that of mucosal mast cells (MMC), and that synthesized
predominantly chondroitin sulfate [35S] proteoglycans. When "MMC-like"
cultured mast cells derived from WBB6F1-+/+ PMC were injected into the
peritoneal cavities of mast cell-deficient WBB6F1- W/Wv mice, the
adoptively transferred mast cell population became 100% berberine
sulfate-positive. In methylcellulose culture, these "second generation PMC"
formed clonal colonies containing both berberine sulfate-positive and
berberine sulfate-negative cells, but exhibited significantly less
proliferative ability than did normal +/+ PMC. Thus, clonal mast cell
populations initially derived from single PMC exhibited multiple and
bidirectional alterations between CTMC-like and MMC-like phenotypes.
However, this process was associated with a progressive diminution of the
mast cells' proliferative ability.
Volume 72,
Issue 3,
pp. 877-885,
09/01/1988
Copyright © 1988 by The American Society of Hematology
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