Human fibroblasts produce granulocyte-CSF, macrophage-CSF, and
granulocyte-macrophage-CSF following stimulation by interleukin-1 and
poly(rI).poly(rC)
WE Fibbe, J Van Damme, A Billiau, N Duinkerken, E Lurvink, P Ralph, BW Altrock, K Kaushansky, R Willemze and JH Falkenburg
Department of Hematology, University Medical Center, Leiden, The
Netherlands.
Electrophoretically pure human interleukin-1 (IL-1) beta was found to
stimulate human fibroblasts in a monolayer culture to elaborate colony-
stimulating activity (CSA). Supernatant fluids from cultures induced with
increasing concentrations of IL-1 were found to stimulate colony formation
of myeloid (CFU-GM), erythroid (BFU-E), and multipotent (CFU- GEMM)
progenitor cells in a dose-dependent fashion. The effect on mixed colony
formation, however, was less than on CFU-GM and BFU-E growth. Similar to
IL-1, the synthetical double-stranded RNA poly(rI).poly(rC) also stimulated
release of CSA by fibroblasts. The kinetics of IL-1- and
poly(rI).poly(rC)-induced CSA release were found to be different, in that
poly(rI).poly(rC)-induced CSA production occurred more slowly. Anti-IL-1
antiserum was able to completely neutralize the IL-1-induced CSA release,
but had no effect on poly(rI).poly(rC)-induced CSF production, suggesting
that the latter effect was mediated by other mechanisms than IL-1 in
supernatant. By the use of specific immunologic assays, G-CSF, M-CSF, and
GM-CSF could be identified in media conditioned by fibroblasts treated with
IL-1 or poly(rI).poly(rC). Poly(rI).poly(rC) appeared to be a better
inducer for M-CSF than IL-1.
Volume 72,
Issue 3,
pp. 860-866,
09/01/1988
Copyright © 1988 by The American Society of Hematology
