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Granulocyte-macrophage colony-stimulating factor enhances selective
effector functions of tissue-derived macrophages
DL Coleman, JA Chodakewitz, AH Bartiss and JW Mellors
Department of Medicine, Yale University School of Medicine, Veterans
Administration Medical Center, West Haven, CT 06516.
Granulocyte-macrophage colony-stimulating factor (GM-CSF) is produced by a
variety of cells at sites of exposure to antigens. GM-CSF has a stimulatory
effect on a number of neutrophil functions, but the effect on macrophage
function is less clear. We investigated the effect of purified murine
recombinant GM-CSF on murine peritoneal macrophage oxidative metabolism,
Fc-dependent phagocytosis, anti-Toxoplasma activity, and expression of
class II major histocompatibility antigen (Iad). GM-CSF significantly
increased phorbol myristate acetate- and zymosan-elicited H2O2 release by
resident and thioglycollate-elicited macrophages after 48 hours in vitro.
The effect of recombinant GM-CSF was blocked by polyclonal anti-GM-CSF
antibody and was not altered by lipopolysaccharide (0.01 to 1.0
microgram/mL). GM-CSF also stimulated Fc-dependent phagocytosis by
peritoneal macrophages, although the stimulation of resident macrophages
(1.4-fold) was less dramatic than that of thioglycollate-elicited cells
(2.1-fold). GM-CSF (at doses up to 100 U/mL) had no effect on macrophage
anti-Toxoplasma activity or on expression of Iad. In addition to
stimulating macrophage growth, GM-CSF selectively promotes the functional
capacity of tissue-derived macrophages.
Volume 72,
Issue 2,
pp. 573-578,
08/01/1988
Copyright © 1988 by The American Society of Hematology
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