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M Akashi, F Takaku, H Nojiri, Y Miura, Y Nagai and M Saito
Division of Hemopoiesis, Institute of Hematology, Tochigi-ken, Japan.
Changes in the composition and metabolism of glycosphingolipid (GSL), which
is one of the cell surface constituents, during cell differentiation of
human T-lymphoblastic leukemia cell line MOLT-3 cells were examined with
special reference to their alterations in E rosette-forming capacity and
expression of surface antigens specific for T-cell lineage. Three molecular
species of neutral GSL and greater than or equal to 13 molecular species of
acidic sialosyl-GSL (ganglioside) were detectable on high-performance
thin-layer chromatography (HPTLC) in untreated MOLT-3 cells. The major
components were ceramide monohexoside and gangliosides GM3 and GD1a. When
the cells were induced by 12-O-tetradecanoyl phorbol 13-acetate (TPA) to
differentiate into more mature T cells, the ganglioside composition changed
distinctively, and the total ganglioside content increased considerably;
mono-, di-, and tri-sialosyl gangliosides concomitantly showed significant
increase, but no new molecular species of GSL specific for the
differentiation were detected. The activity of one sialyltransferases,
CMP-sialic acid:CDH sialyltransferase, which synthesizes ganglioside GM3
and the total sialic acid content of the cell surface, parallelled the
extent of cell differentiation. Examination of another human
T-lymphoblastic leukemia cell line, HPB- ALL, indicated that TPA could also
induce the cells to differentiate along T-cell lineage and that changes in
the ganglioside pattern during differentiation are similar to those of
MOLT-3 cells. The results indicate that human T-lymphoid cell
differentiation intimately involves elongation of neutral
oligosaccharide-moieties and the addition of sialic acid residues to
gangliosides, resulting in more mature T cells containing higher
gangliosides. Both the sialyltransferase activity and the sialic acid
content, as well as the ganglioside pattern, might be new biochemical
markers specific for human T-lymphoblastic cell differentiation.
This article has been cited by other articles:
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| Copyright © 1988 by American Society of Hematology Online ISSN: 1528-0020 | |||||||||
