|
|
 Previous Article | Table of Contents | Next Article 
Functional and morphological differentiation induction of a human
megakaryoblastic leukemia cell line (MEG-01s) by phorbol diesters
M Ogura, Y Morishima, M Okumura, T Hotta, S Takamoto, R Ohno, N Hirabayashi, H Nagura and H Saito
First Department of Internal Medicine, Nagoya University School of
Medicine, Japan.
The effect of differentiation induction by a tumor-promoting phorbol
diester, 12-O-tetradecanoylphorbol-13-acetate (TPA) on a clonal human
megakaryoblastic cell line, MEG-O1s, was investigated, and a prominent
response was demonstrated. The cells became weakly adherent, developed
conspicuous cytoplasmic blebs, and displayed mature megakaryocytic
characteristics by light microscopy such as the development of azurophilic
cytoplasmic granules and a mosaic pattern of oxyphilic patches,
multiplication of nuclei, and enhancement of the PAS reaction and
alpha-naphthyl acetate esterase staining. Ultrastructural studies
demonstrated the development of prominent cytoplasmic blebs, budding of
blebs, and multiplication of nuclei. Numerous granules with central
nucleoids that are similar to alpha granules had developed as well as
granules with high electron density and clearly demarcated zones. Surface
marker analysis revealed a moderate increase in IgG Fc receptor levels and
a profound decrease in C3 receptor sites. By an immunofluorescent technique
using monoclonal and polyclonal antibodies, a dramatic change in the
expression of several megakaryocyte-platelet- specific proteins was
demonstrated. All the proteins that had been expressed before induction
such as platelet glycoprotein (GP) IIb/IIIa, fibrinogen, von Willebrand
factor, factor XIIIA, beta-thromboglobulin (beta-TG), and HLA class 1
antigen were profoundly enhanced after induction by TPA. Induction by TPA
led to the expression of fibronectin and factor V, which were not detected
on nontreated cells. An ultrastructural immunoperoxidase study demonstrated
platelet GPIb and GPIIb/IIIa in both plasma membranes and protein synthesis
areas such as perinuclear cisternae and endoplasmic reticulum after TPA
induction. beta-TG was also observed in some cytoplasmic granules of
TPA-treated cells. TPA remarkably increased the secretion of beta-TG into
the culture medium of MEG-01s. Ploidy was also increased from 2C to 4C to
4C to 8C. Similar maturation of MEG-01s was induced by other phorbol
diester analogues such as phorbol-12,13-dibutyrate, but not by phorbol
itself. These results indicate that phorbol diester, TPA, can bring about
differentiation and maturation of a human megakaryoblastic cell line
(MEG-01s) and that MEG-01s cells will provide a useful model for studying
megakaryocytic differentiation and numerous megakaryocyte-
platelet-specific proteins.
Volume 72,
Issue 1,
pp. 49-60,
07/01/1988
Copyright © 1988 by The American Society of Hematology
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
J. L. Granick, D. V. Reneer, J. A. Carlyon, and D. L. Borjesson
Anaplasma phagocytophilum infects cells of the megakaryocytic lineage through sialylated ligands but fails to alter platelet production
J. Med. Microbiol.,
April 1, 2008;
57(4):
416 - 423.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Duquette and O. Laneuville
Translational Regulation of Prostaglandin Endoperoxide H Synthase-1 mRNA in Megakaryocytic MEG-01 Cells. SPECIFIC PROTEIN BINDING TO A CONSERVED 20-NUCLEOTIDE CIS ELEMENT IN THE 3'-UNTRANSLATED REGION
J. Biol. Chem.,
November 15, 2002;
277(47):
44631 - 44637.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. Faraday, J. J. Rade, D. C. Johns, G. Khetawat, S. J. Noga, J. F. DiPersio, Y. Jin, J. L. Nichol, J. S. Haug, and P. F. Bray
Ex Vivo Cultured Megakaryocytes Express Functional Glycoprotein IIb-IIIa Receptors and Are Capable of Adenovirus-Mediated Transgene Expression
Blood,
December 15, 1999;
94(12):
4084 - 4092.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Taniguchi, H. Endo, N. Chikatsu, K. Uchimaru, S. Asano, T. Fujita, T. Nakahata, and T. Motokura
Expression of p21Cip1/Waf1/Sdi1 and p27Kip1 Cyclin-Dependent Kinase Inhibitors During Human Hematopoiesis
Blood,
June 15, 1999;
93(12):
4167 - 4178.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. G. Genever, D. J.P. Wilkinson, A. J. Patton, N. M. Peet, Y. Hong, A. Mathur, J. D. Erusalimsky, and T. M. Skerry
Expression of a Functional N-Methyl-D-Aspartate-Type Glutamate Receptor by Bone Marrow Megakaryocytes
Blood,
May 1, 1999;
93(9):
2876 - 2883.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. Maulon, S. Guérin, J. E. Ricci, Y. Colin, J. P. Cartron, and P. Auberger
Differential expression of the Kell blood group and CD10 antigens: two related membrane metallopeptidases during differentiation of K562 cells by phorbol ester and hemin
FASEB J,
May 1, 1998;
12(7):
531 - 539.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
N. Meller, A. Altman, and N. Isakov
New Perspectives on PKC{theta}, a Member of the Novel Subfamily of Protein Kinase C
Stem Cells,
May 1, 1998;
16(3):
178 - 192.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
Z. Cui, M. P. Reilly, S. Surrey, E. Schwartz, and S. E. McKenzie
-245 bp of 5'-Flanking Region From the Human Platelet Factor 4 Gene Is Sufficient to Drive Megakaryocyte-Specific Expression In Vivo
Blood,
April 1, 1998;
91(7):
2326 - 2333.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Uchimaru, T. Taniguchi, M. Yoshikawa, S. Asano, A. Arnold, T. Fujita, and T. Motokura
Detection of Cyclin D1 (bcl-1, PRAD1) Overexpression by a Simple Competitive Reverse Transcription-Polymerase Chain Reaction Assay in t(11; 14)(q13; q32)-Bearing B-Cell Malignancies and/or Mantle Cell Lymphoma
Blood,
February 1, 1997;
89(3):
965 - 974.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
