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This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Williams, D. A. Articles by Dexter, T. M. Search for Related Content PubMed PubMed Citation Articles by Williams, D. A. Articles by Dexter, T. M. Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  Restriction of expression of an integrated recombinant retrovirus in primary but not immortalized murine hematopoietic stem cells DA Williams, B Lim, E Spooncer, J Longtine and TM Dexter Division of Hematology-Oncology, Howard Hughes Medical Institute, Boston, MA. A recombinant retrovirus (DHFR*-SVADA) in which human adenosine deaminase (ADA) cDNA is transcribed from an internal SV40 promoter was used to infect murine hematopoietic stem and progenitor cells. Human ADA enzyme was not expressed in infected primary murine pluripotent stem cell-derived spleen or progenitor colonies (CFU-GM, CFU-Mix, BFU- E). In contrast, human ADA enzyme activity was readily detected in progenitor colonies derived from immortalized multipotent factor- dependent cells. The level of human enzyme was near endogenous murine enzyme levels and was equivalent in undifferentiated stem cells and differentiated myeloid, erythroid, and mixed colonies. These results indicate that cellular properties other than the stage of differentiation are important in determining the expression of foreign sequences introduced by retroviruses. Cell lines that are immortalized but still capable of induced differentiation may contain factors that abrogate blocks to expression that are manifested in primary hematopoietic stem cells. Volume 71, Issue 6, pp. 1738-1743, 06/01/1988 Copyright © 1988 by The American Society of Hematology CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: J. Zwiebel, S. Freeman, P. Kantoff, K Cornetta, U. Ryan, and W. Anderson High-level recombinant gene expression in rabbit endothelial cells transduced by retroviral vectors Science, January 13, 1989; 243(4888): 220 - 222. [Abstract] [PDF]

	Copyright © 1988 by American Society of Hematology         Online ISSN: 1528-0020