A myeloid-related sequence that localizes to human chromosome 8q21.1-22
WM Mars, P van Tuinen, HA Drabkin, JW White and GF Saunders
Department of Biochemistry and Molecular Biology, University of Texas, M.D.
Anderson Hospital and Tumor Institute, Houston 77030.
A myeloid-related sequence (mrs) has previously been identified that is
highly expressed in selected subpopulations of myeloid leukocytes.
Nucleotide sequence analysis indicates that mrs encodes what is apparently
a unique 93-amino acid protein that includes an 18-amino acid leader
sequence. Hybridization of an mrs cDNA probe to a Southern blot made from
somatic cell hybrid DNAs shows 100% concordance with human chromosome 8,
thus indicating that mrs localizes to this chromosome. In situ
hybridization to metaphase chromosomes further sublocalizes mrs to bands
8q21.1-23 as 58% of the grains displayed on chromosome 8 were clustered in
this region. This area encompasses the translocation breakpoint 8q22, which
is rearranged in an estimated 18% of patients diagnosed with the M2
subclassification of acute nonlymphocytic leukemia (M2-ANLL). When Southern
blot hybridization was performed by using somatic cell hybrid DNAs
harboring either a single 8q- or a single 21q+ chromosome from two
different patients with M2- ANLL, a signal was only detected in the hybrid
containing the 8q- chromosome.
Volume 71,
Issue 6,
pp. 1713-1719,
06/01/1988
Copyright © 1988 by The American Society of Hematology
