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Reduction in pO2 decreases the fibrinolytic potential of cultured bovine
endothelial cells derived from pulmonary arteries and lung microvasculature
J Wojta, RL Jones, BR Binder and RL Hoover
Department of Pathology, Vanderbilt University, Nashville, TN 37232.
The effect of anoxia on the fibrinolytic potential of cultured endothelial
cells derived from bovine pulmonary artery and bovine lung microvasculature
was studied. Both cell types reacted with an increase in plasminogen
activator inhibitor (PAI) activity and a decrease in the plasminogen
activator (PA) activity in the media after incubation under anoxic
conditions. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis
followed by fibrin autography and reverse fibrin autography indicated that
the change in fibrinolytic potential was due to an impaired release of PA
and not an increase in the production of PAI. Although anoxia did not
affect the viability of the cells as judged by 51Cr release, their
metabolism was influenced, which is reflected by increases in the levels of
lactate in cell lysates and media. Furthermore, the effect of short-term
anoxia on PA and PAI could not be reversed by reoxygenation for 24 hours.
The results are discussed in terms of helping to explain the tendency of
reocclusion after successful thrombolytic therapy, the development of
pulmonary hypertension, and the thrombotic tendency of areas with an
impaired circulatory supply.
Volume 71,
Issue 6,
pp. 1703-1706,
06/01/1988
Copyright © 1988 by The American Society of Hematology
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