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Recombinant gibbon interleukin-3 acts synergistically with recombinant
human G-CSF and GM-CSF in vitro
RL Paquette, JY Zhou, YC Yang, SC Clark and HP Koeffler
Department of Medicine, UCLA Medical Center.
Recombinant gibbon interleukin-3 (IL-3) is a multilineage hematopoietic
colony-stimulating factor (CSF) that recently was cloned and found to be
highly homologous with human IL-3. Gibbon IL-3, as well as human
granulocyte-CSF (G-CSF) and human granulocyte-macrophage CSF (GM-CSF),
stimulated normal human bone marrow cells to form myeloid colonies in soft
agar in a sigmoidal dose-response manner. When IL-3 was added to increasing
concentrations of G-CSF or GM-CSF, synergistic colony formation occurred as
compared with the effects of each CSF alone. Synergism was also noted when
G-CSF was added with GM-CSF and when all the CSFs were added
simultaneously. The combination of IL-3 and GM-CSF was less stimulatory
than all the other CSF combinations. At day 11 of culture, IL-3 induced
granulocyte-macrophage (38%), eosinophil (30%), granulocyte (18%), and
macrophage (14%) colony formation. In summary, gibbon IL-3 is a growth
factor that can synergize with other CSFs to enhance proliferation of
myeloid-committed progenitors, suggesting that combinations of CSFs may
have clinical utility in patients with neutropenia of various etiologies.
Volume 71,
Issue 6,
pp. 1596-1600,
06/01/1988
Copyright © 1988 by The American Society of Hematology
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