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Defective helper function of purified T4 cells and excessive suppressor
activity of purified T8 cells in patients with B-cell chronic lymphocytic
leukemia. T4 suppressor effector cells are present in certain patients
JE Kunicka and CD Platsoucas
Memorial Sloan-Kettering Cancer Center, New York.
We investigated helper and suppressor functions to B-cell responses and T-T
cell interactions of purified T4 and T8 cells from 20 untreated patients
with B-cell chronic lymphocytic leukemia (CLL). Appropriate mixtures of
purified T4 or T8 cells from patients with CLL were cultured with purified
B cells or T4 and B cells from normal donors for 7 days with pokeweed
mitogen (PWM). IgM, IgA, and IgG produced were determined in the
supernatants of these cultures by a heavy chain- specific enzyme-linked
immunosorbent assay (ELISA) and compared to those obtained by the
corresponding mixtures of T4, T8, and B cells from normal donors. Purified
T4 cells from 14 of 20 patients with CLL exhibited defective helper
function (P less than .001) to immunoglobulin (Ig) production by purified B
cells from normal donors. Purified T4 cells from 6 of these 14 patients
were able to suppress significantly (P less than .001) and in a
concentration-dependent manner Ig production by mixtures of T4 and B cells
from normal donors, in the absence of T8 cells. These suppressor effector
T4 cells from certain patients were partially radiosensitive. Purified T8
cells from 8 of 20 patients with CLL exhibited excessive suppressor
activity. These cells significantly suppressed (P less than .001), Ig
production by mixtures of T4 and B cells from normal donors to a degree
significantly higher (P less than .005) than that observed by equal numbers
of T8 cells from normal donors. This inhibition was dependent on the
numbers of the T8 CLL cells added to the cultures. Excessive suppressor
activity by T8 CLL cells was at least in part radiosensitive in four of
eight patients. These results demonstrate a wide range of immunoregulatory
T-cell abnormalities in patients with CLL. Naturally occurring T4
suppressor effector cells, directly inhibiting Ig production by mixtures of
T4 and B cells, in the absence of T8 cells, are present in certain patients
with CLL.
Volume 71,
Issue 6,
pp. 1551-1560,
06/01/1988
Copyright © 1988 by The American Society of Hematology
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