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This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Cai, S. P. Articles by Kan, Y. W. Search for Related Content PubMed PubMed Citation Articles by Cai, S. P. Articles by Kan, Y. W. Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  A simple approach to prenatal diagnosis of beta-thalassemia in a geographic area where multiple mutations occur SP Cai, JZ Zhang, DH Huang, ZX Wang and YW Kan Department of Medicine, Howard Hughes Medical Institute, University of California, San Francisco 94143-0724. We describe a simple approach for detecting beta-thalassemia mutations in geographic areas such as southern China where multiple mutations are known to occur. Segments of the beta-globin gene were amplified in vitro by using the polymerase chain reaction. Dot blot hybridization of the amplified DNA with oligonucleotide probes corresponding to the six mutations found in southern China could directly identify the mutations causing beta-thalassemia in the affected families. The increased number of target sequences after amplification allows the use of 35S-labeled probes, which are reusable for up to 3 months. The mutations can be determined in two days. Volume 71, Issue 5, pp. 1357-1360, 05/01/1988 Copyright © 1988 by The American Society of Hematology CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: W. Wang, S. K.Y. Kham, G.-H. Yeo, T.-C. Quah, and S. S. Chong Multiplex Minisequencing Screen for Common Southeast Asian and Indian {beta}-Thalassemia Mutations Clin. Chem., February 1, 2003; 49(2): 209 - 218. [Abstract] [Full Text] [PDF]

	Copyright © 1988 by American Society of Hematology         Online ISSN: 1528-0020