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This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Roussel, M. F. Articles by Sherr, C. J. Search for Related Content PubMed PubMed Citation Articles by Roussel, M. F. Articles by Sherr, C. J. Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  Introduction of a human colony stimulating factor-1 gene into a mouse macrophage cell line induces CSF-1 independence but not tumorigenicity MF Roussel, CW Rettenmier and CJ Sherr Department of Tumor Cell Biology, St. Jude Children's Research Hospital, Memphis, TN 38105. The SV40-immortalized mouse macrophage cell line, BAC1.2F5, is strictly dependent on CSF-1 for its survival and proliferation in culture. Introduction of a retroviral vector containing a 1.6 kilobase (kb) pair human CSF-1 cDNA into these cells abrogated their growth factor dependence but did not render the cells tumorigenic in nude mice. The infected macrophages contained multiple copies of the vector provirus, expressed both membrane-bound and secreted forms of CSF-1, and exhibited constitutive down modulation of the murine CSF-1 receptor. Because insertion of the v-fms gene has previously been shown to abrogate factor dependence and induce tumorigenicity in BAC1.2F5 macrophages, the failure of these cells to express a fully transformed phenotype after persistent stimulation by endogenous CSF-1 suggests that the v-fms and c-fms gene products provide different signals for cell proliferation. Volume 71, Issue 5, pp. 1218-1225, 05/01/1988 Copyright © 1988 by The American Society of Hematology CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: C. J. Sherr, C.W. Rettenmier, and M.F. Roussel Macrophage Colony-stimulating Factor, CSF-1, and Its Proto-oncogene-encoded Receptor Cold Spring Harb Symp Quant Biol, January 1, 1988; 53(0): 521 - 530. [Abstract] [PDF]

	Copyright © 1988 by American Society of Hematology         Online ISSN: 1528-0020