Effects of hematopoietin-1 and interleukin 1 activities on early
hematopoietic cells of the bone marrow
KM Zsebo, J Wypych, VN Yuschenkoff, H Lu, P Hunt, PP Dukes and KE Langley
Amgen, Thousand Oaks, CA 91320.
Hematopoietin-1 (H-1) was purified from the human cell line 5637 and two
amino acid sequences were observed in the preparation. One sequence was
identical to that of interleukin 1 alpha (IL 1 alpha) and the other to that
of IL 1 beta. The action of recombinant IL 1 alpha and other hematopoietic
growth factors was studied using (a) a high proliferative potential
colony-forming cell assay that uses primitive hematopoietic precursors from
bone marrow, and (b) a spleen colony-forming unit assay. The results
indicate that the IL 1 alpha target cell population is different than the
target cell populations of IL 3, granulocyte- macrophage colony-stimulating
factor; that IL 1 alpha in combination with mononuclear phagocyte
colony-stimulating factor provides a proliferative stimulus; and that IL 1
alpha has at least a survival- enhancing and possibly
proliferation-inducing effect on primitive hematopoietic stem cells.
Volume 71,
Issue 4,
pp. 962-968,
04/01/1988
Copyright © 1988 by The American Society of Hematology
