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Mechanism of transient adsorption of fibrinogen from plasma to solid
surfaces: role of the contact and fibrinolytic systems
JL Brash, CF Scott, P ten Hove, P Wojciechowski and RW Colman
Department of Chemical Engineering, McMaster University, Hamilton, Ontario,
Canada.
The transient detection of fibrinogen on surfaces has been described
(Vroman effect) and high-mol-wt kininogen (HK) has been shown to play a
role in this reaction. In this study, we attempted to identify the form of
HK responsible for preventing detection of the fibrinogen initially
adsorbed from plasma to various artificial surfaces and to determine if
other plasma components were involved. We compared 125I-fibrinogen
adsorption in the presence of normal plasma to plasma deficient in specific
proteins. On all surfaces tested, we found that fibrinogen was displaced
from the surface. The extent of displacement was greatly reduced, however,
but not eliminated in HK-deficient plasma. Factor XII- deficient plasma
also showed reduced fibrinogen displacement. These data indicate that HK
can actually displace fibrinogen; however, factor XII, or a factor
XII-mediated reaction also appears to be necessary for this displacement to
occur. Furthermore, when normal plasma was first subjected to extensive
contact activation by dextran sulfate, during which the HK was extensively
degraded to components smaller than the light chain (as assessed by Western
blotting), we observed greatly reduced displacement of fibrinogen.
Extensive contact activation of Factor XI-deficient plasma failed to show
low-mol-wt derivatives, however, and displacement of fibrinogen was similar
to normal plasma that had not undergone extensive activation. These data
indicate that HKa (active cofactor produced during contact activation by
factor XIIa or kallikrein) is primarily responsible for displacing
fibrinogen, and that HKi (inactive cofactor generated by factor XIa) cannot
displace fibrinogen. The fibrinogen from all plasma samples looked similar
by Western blot analysis, suggesting that fibrinogenolysis was not a
component of the Vroman effect. In addition, experiments performed with
plasma prechromatographed on lysine agarose showed that a lysine- agarose
adsorbable protein may be minimally involved in fibrinogen desorption and a
synergism may exist between HK and that protein.
Volume 71,
Issue 4,
pp. 932-939,
04/01/1988
Copyright © 1988 by The American Society of Hematology
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