Vasoactive amines directly modify endothelial cells to affect
polymorphonuclear leukocyte diapedesis in vitro
J Doukas, D Shepro and HB Hechtman
Bovine aortic endothelial cells were cultured on the basement membrane
surface of amnionic membrane and used as a substrate for polymorphonuclear
leukocyte (PMN) diapedesis in vitro. Norepinephrine (NE), serotonin (5HT),
or phalloidin treatment of the endothelial cells (ECs) reduces, whereas
histamine or cytochalasin B increases, the number of PMNs migrating across
the ECs and amnionic membrane. In contrast, amine treatment of PMNs or
acellular amnionic membrane does not alter PMN diapedesis or chemotaxis.
The NE and histamine effects are blocked by appropriate receptor
antagonists, but the 5HT effect is not. All the agents' effects are also
reversible. Qualitatively similar effects on EC permeability to Evan's
blue-labeled albumin occur with all agents; however, PMN adhesion to ECs is
not affected. Previously, we reported that NE and 5HT increase stress fiber
numbers and decrease EC permeability to macromolecules in vitro, whereas
histamine has the opposite effects, and that NE and 5HT eliminate the
erythrocyte extravasation associated with thrombocytopenia in vivo. In this
study, we propose that these vasoactive amines also alter PMN diapedesis in
vitro through a direct effect on the EC, in part due to alterations in the
EC cytoskeleton.
Volume 69,
Issue 6,
pp. 1563-1569,
06/01/1987
Copyright © 1987 by The American Society of Hematology
