|
|
 Previous Article | Table of Contents | Next Article 
Establishment and characterization of a cell line, TOM-1, derived from a
patient with Philadelphia chromosome-positive acute lymphocytic leukemia
M Okabe, S Matsushima, M Morioka, M Kobayashi, S Abe, K Sakurada, M Kakinuma and T Miyazaki
A new Philadelphia chromosome (Ph1)-positive cell line, designated TOM- 1,
was derived from bone marrow cells of a patient with Ph1-positive acute
lymphocytic leukemia (ALL). The TOM-1 cells were positive for Ia and B1
antigens and terminal deoxynucleotidyl transferase (TdT) but negative for
common ALL antigen. Although neither surface Ig nor cytoplasmic Ig was
detected, the TOM-1 cells contained rearranged immunoglobulin-H chain genes
but retained germ-line kappa chain and germ-line T cell receptor beta-chain
genes. These results indicate that the TOM-1 cells reside as the progenitor
of pre-B cells. We have investigated the chromosome 22 breakpoint and c-abl
gene expression in the TOM-1 cells. We found that the breakpoint on
chromosome 22 was within the breakpoint cluster region (bcr) in the TOM-1
cells. We also found the breakpoints within or near bcr in four of six
Ph1-positive ALL cases, similar to the findings in Ph1-positive CML cases.
Amplification of the c-abl gene was not detected in the TOM-1 cells. The
leukemic cells isolated from a patient with CML in myeloid crisis contained
a novel 8-kilobase (kb) abl-related messenger RNA (mRNA), but the TOM-1
cells contained c-abl transcripts of only normal sizes, despite the fact
that they showed the bcr gene rearrangement.
Volume 69,
Issue 4,
pp. 990-998,
04/01/1987
Copyright © 1987 by The American Society of Hematology
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
E. Laurent, M. Talpaz, H. Kantarjian, and R. Kurzrock
The BCR Gene and Philadelphia Chromosome-positive Leukemogenesis
Cancer Res.,
March 1, 2001;
61(6):
2343 - 2355.
[Full Text]
|
|
|
|
|
|
|
G. J.A. ten Bosch, J. H. Kessler, A. M. Joosten, A. A. Bres-Vloemans, A. Geluk, B. C. Godthelp, J. van Bergen, C. J.M. Melief, and O. C. Leeksma
A BCR-ABL Oncoprotein p210b2a2 Fusion Region Sequence Is Recognized by HLA-DR2a Restricted Cytotoxic T Lymphocytes and Presented by HLA-DR Matched Cells Transfected With an Iib2a2 Construct
Blood,
August 1, 1999;
94(3):
1038 - 1045.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. Takeda, M. Shibuya, and Y. Maru
The BCR-ABL oncoprotein potentially interacts with the xeroderma pigmentosum group B protein
PNAS,
January 5, 1999;
96(1):
203 - 207.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. Gauthier, B. Lemmers, V. Guelpa-Fonlupt, M. Fougereau, and C. Schiff
{micro}-Surrogate Light Chain Physicochemical Interactions of the Human PreB Cell Receptor: Implications for VH Repertoire Selection and Cell Signaling at the PreB Cell Stage
J. Immunol.,
January 1, 1999;
162(1):
41 - 50.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H.-C. Aasheim, L. W.M.M. Terstappen, and T. Logtenberg
Regulated Expression of the Eph-Related Receptor Tyrosine Kinase Hek11 in Early Human B Lymphopoiesis
Blood,
November 1, 1997;
90(9):
3613 - 3622.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
