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1 Department of Hematology, Division of Medicine, Walter Reed Army Institute of Research, Washington, D. C.
Using paper and starch-gel electrophoresis we have demonstrated binding
of hemoglobin, myoglobin and hematin by human serum protein which is not
a haptoglobin. This protein was present in all sera tested except that from
two patients with hemolytic anemia and was also present in serum genetically
deficient in haptoglobin. The binding capacity of this protein is low and contributes only slightly to
the total hemoglobin binding capacity of normal serum. However, it may
represent most or all of the binding in sera from patients with hemolytic
disease.
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