Blood, 1960, Vol. 16, No. 4, pp. 1491-1498.
© 1960 American Society of Hematology, Inc.
The Cytologic Reaction of the Spleen
in Experimental Amyloidosis in Mice
AVINOAM ZLOTNICK 1 and
CHLOE TAL 1
1 Department of Internal Medicine A and the Department of Nervous Diseases,
Laboratory of Experimental Neurology, The Rothschild-Hadassah University Hospital and
the Hebrew University-Hadassah Medical School, Jerusalem, Israel.
Seventy W.-H. Swiss line mice were injected with adjuvant, TAB vaccine
and TAB vaccine plus adjuvant. The 61 mice which survived form the subject of this study.
Spleen smears and histologic sections taken after completing immunization
revealed a marked deposition of amyloid in 48 of the animals. Varying degrees of plasma cell hyperplasia were observed in 46 animals, and increased
granulopoiesis in 37. In 28 of the amyloid-bearing animals some of the plasma
cells were transformed into "flame cells" which stained faintly positively
with PAS and metachromatically with methyl violet.
Electrophoresis of the pooled sera of animals receiving adjuvant alone,
TAB plus adjuvant or TAB alone, compared to normal controls, showed decrease in albumine and an increase in the globulin fraction. Animals receiving
adjuvant alone showed an increase in the beta globulins, while animals receiving TAB plus adjuvant or TAB alone showed an increase in the gamma
globulins.
The relationship between the production of amyloid, plasma cell hyperplasia and the appearance of "flame cells" is discussed.
Submitted on February 16, 1960
Accepted on March 24, 1960
