Blood, 1960, Vol. 16, No. 4, pp. 1456-1468.
© 1960 American Society of Hematology, Inc.
Studies on Agranulocytosis. II. The Effect of
Chlorpromazine upon the Influx of S35
L-Cystine and L-Methionine into
Human Leukocytes
ANTHONY V. PISCIOTTA 1,
SHIRLEY N. EBBE 1,
MARY DALY 1,
MONA RUWALDT 1,
MILTON GLASER 1, and
GLORIA O. METZGER 1
1 Blood Research Laboratory, Department of Medicine, Marquette University
School of Medicine and the Milwaukee County General Hospital, Milwaukee, Wis.
1. When whole blood was incubated in vitro with S-35 L-cystine and L-methionine, the blood cells became radioactive.
2. Preincubation of whole blood from normals and from patients susceptible
to agranulocytosis with chlorpromazine showed no effect upon uptake of S-35
L-cystine and L-methionine by leukocytes.
3. The in vivo administration of S-35 L-cystine was followed by the appearance of radioactive leukocytes. Peak radioactivity occurred in leukocytes in 5
to 12 days.
4. Pretreatment of test subjects with large doses of chlorpromazine did not
block the uptake of S-35 L-cystine by leukocytes in vivo. Leukocytes of women
showed an increase in the incorporation of S-35 L-cystine, in vivo. Studies
performed in vivo on two persons during recovery from agranulocytosis
showed enhanced uptake of L-cystine in one and a normal uptake in the other.
Submitted on March 24, 1960
Accepted on July 13, 1960
